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Title of the project |
Brief objectives |
| National Citrus repository of NRCC
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i) Collection of germplasm of rootstocks, mandarins, acid lime, pummelo, grapefruit and sweet oranges.
ii) Evaluation of germplasm in order to characterize and catalogue them according to IBPGR Descriptors for Citrus.
iii) Screening of rootstocks germplasm against Phytopthora and citrus nematode.
iv) Screening of germplasm for different viral and bacterial pathogens
v) Micropropogation of citrus rootstocks
vi) Shoot tip grafting of exotic germplasm.
vii) Evaluation of hybrid seedlings of rootstocks.
viii) Evaluation of irridiated seedlings for Nagpur mandarin
ix) To select and evaluate the desirble clones of Nagpur mandarin, Khasi mandarin, Coorg mandarin, Sweet orange and acid lime having early/late maturity, seedless/less seeded fruits, high T.S.S. and spreading type of plants. |
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Production of disease free Sweet orange (Citrus sinensis Osbeck) plants by STG
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i) To produce true to type and disease-free planting material of Mosambi.
ii) Multiplication of disease free planting material of Mosambi.
iii) To establish mother block of disease-free sweet oranges orchards using STG technique.
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| Development of in vitro regeneration and genetic transformation strategies for citrus improvement |
- To standardize somatic embryognesis based in vitro regeneration protocol for Citrus reticulata.Blanco
- To develop agro-bacterium transformation protocol for citrus genetic transformation and to confirm transgenic gene expression
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| Evaluation of citrus rootstock strains for optimum growth and productivity of Nagpur mandarin and Acid lime

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i) To study the compatibility of different citrus root stock strains with Nagpur mandarin and Acid lime.
ii) To determine the effect of strains of rootstock on growth, flowering, fruiting, productivity and fruit quality.
iii) To study root spread and nutrients uptake pattern of different rootstock strains. |
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Induction and regulation of flowering in Nagpur mandarin and Acid lime

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i) To determine the optimum quantum of water stress required for induction of flowering in Nagpur mandarin .
ii) To study the effect of different growth substances on induction of flowering in acid lime.
iii) To regulate cropping in Nagpur mandarin and acid lime.
iv) To study the fruit yield, quality and longevity of citrus orchards practising Ambia and Mrig flowering. |
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Orchard
efficiency of Nagpur mandarin in Central
India
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i) To find out the factors influencing the citrus production.
ii) To find out the relative importance of the factors, which affect the citrus production.
iii) To find out the critical importance of the factors responsible for yield variation. |
| Organic manuring in Nagpur mandarin (Citrus reticulata Blanco)
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i) Quantitative estimation of organic substrate for Nagpur mandarin
ii) Nutrient supplying capacity of various organic substrate
iii) Comparisons of organic versus inorganic fertilization in terms of yield, quality and post harvest life. |
| Micronutrient management for optimum growth, yield and quality of Nagpur mandarin (Citrus reticulata Blanco)
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i) Comparative efficacy of different methods of fertilizer application.
ii) Optimum fertilizer requirement vis-a –vis on growth, yield and fruit quality
iii) Micronutrient application and changes in soil-plant nutrition.
iv) Development of sustainable micronutrient amelioration strategy to address constraint free production.
v) Changes is soil available pool of nutrients in response to fertilization. |
| Rain water management in water shed based Citrus orchards
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i) To explore the feasibility of rain water use in Nagpur mandarin through micro irrigation
ii) To find out the water requirement of young Nagpur mandarin plants infield conditions under drip irrigation.
iii) To study the efficacy of in-situ soil and water conservation measures in Nagpur mandarin and acid lime grown in sloppy lands.

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| Study on adoption of improved Technology by Nagpur mandarin growers |
i) To know the knowledge level of mandarin growers about the recommended improved technology
ii) To understand the constraints in adoption of select technology. |
| Nursery Management in Citrus
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i) To reduce the time required for propagation of Nagpur mandarin and acid lime by using conventional method of grafting.
ii) To standardize the nutritional requirements of nursery plants
iii) To standardize the biofertilizer requirements of citrus.
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| Studies on citrus based farming system

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i) To evaluate citrus based integrated farming systems
ii) To evaluate multi-species mixed cropping systems
iii) To evaluate various alternative fruit crops vis a vis Nagpur mandarin.
iv) To work out the economics of various fruit crop as an alternative to citrus.
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| Studies on bio-intensive management of insect pests in citrus

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i) To evaluate bio-agents against insect pest under field condition.
ii) To develop bio-intensive modules for citrus insect pest management. |
| Evaluation of certain bio-pesticides against citrus blackfly, psylla and leaf miner.
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i) To evaluate bio-pesticides against blackfly, psylla and leaf miner.
ii) To identify Oviposition preference by leaf miner, blackfly and psylla with reference to leaf age.
iii) To determine the selectivity of bio-pesticides against bio-agents.
iv) To study the phytotoxicity of bio-pesticides on citrus.
v) To find out the insecticide residue on citrus fruits.
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| Diagnosis and Management of bacterial diseases infecting Citrus
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i) Survery and collection of different Xanthomonas axonopodis pv.citri (Xac) isolates and greening isolates from various regions of the country.
ii) Development of PCR-based technology for detection of greening bacterium
iii) Molecular characterization of Xac isolates to detect and differentiate its strains
iv) Management of bacterial canker disease by
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Using new chemicals
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Using biological control measures
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| Integrated management of Citrus nematode

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i) To survey and identify phytonematodes in citrus nurseries and orchards.
ii) To study population dynamic of parasitic nematodes in Nagpur mandarin and acid lime orchards.
iii) To find out optimum doses and application times of suitable nematicides for the management of Tylenchulus semipenetrans in Nagpur mandarin orchards.
iv) To find out efficacy of certain oil cakes in the management of Tylenchulus semipenetrans infecting acid lime. |
| Study in pre and post-harvest physiology of citrus fruits

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i) To standardize external and internal quality and harvesting maturity for ‘Ambia’ (Spring blossom) crop of Mosambi orange, khasi mandarin and Sathguid orange.
ii) To develop and evaluate packaging containers.
iii) To collect information on size, shape, colour, blemishes and other quality preference about citrus fruits in different importing countries.
iv) To develop MAP System.
v) To develop eco-friendly non-hazardous pre and post harvest treatment to extend shelf life/storage life.
vi) To develop food grade-wx coating/edible coatings.

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| Studies on waste utilization and value addition of Nagpur mandarin

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i) To standardize the different stages for optimum production of by-products.
Ii) To quantify the bitter principle and develop the debettering techniques for improvement of juice quality in Nagpur mandarin.
iii) To study the condition for clarification and concentration of citrus juice by membrane processing.
iv) To extract and develop the value added products (Essential oil, Pectin, Phenols and animal feed etc) from citrus waste. |
| Molecular marker based Analysis of Citrus Genetic Diversity
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i) Fingerprinting of different citrus accessions for efficient maintenance of the germplasm repository.
ii) Genetic diversity analysis of citrus germplasm for establishing phylogenetic relationships.

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| Automatic irrigation scheduling and fertigation through microjet irrigation system in Nagpur mandarin (Citrus reticulata Blanco

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i) To study the automatic irrigation scheduling with drip irrigation system in Nagpur mandarin
ii) To study the effect of different micro-jet irrigation systems on plant growth, fruit growth rate, quality and yield of Nagpur mandarin
iii) To study the soil moisture distribution patter under different micro-jet irrigation systems
iv) To standardize the capacity ad arrangement of the emitters for better uniformity of water distribution in Nagpur mandarin
v)To study the soil moisture distribution pattern different irrigation arrangements
vi) To evaluate the K fertigation using different potash fertilizer under micro-jet irrigation system in Nagpur mandarin
vii) To standardize the dose of potash (K) using fertigation in Nagpur mandarin
viii) To study the economic feasibility of auto-drip irrigation, micro-jet irrigation and K fertigation on production of Nagpur mandarin. |
| Diagnostics of emerging plant viruses

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i) Development of ELISA, PCR and NASH based diagnostics against CTV
ii) Validation of diagnostics for field use. |
| Commercial production of disease free Citrus plants - Revolving Fund

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i) To raise disease free nursery plants of Nagpur mandarin, acid lime and sweet orange for commercial purpose.
ii) To generate resources for the Centre.
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| Large scale production and demonstration of virus free planting material by micrografting in Nagpur mandarin – DBT funded
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i) Large scale multiplication, raising of foundation block of STG and demonstration by conducting training
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